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7The signal to noise ratio is the amount of an observed effect due to the intended process compared to other “background noise”. In this case, light resulting from nucleotide addition versus other chemical reactions. Luciferase recognizes and acts on dATP in solution, creating a spurious signal unrelated to nucleotide addition, which must be accounted for during analysis. Fortunately, the modified “A” nucleotide effectively suppresses this side reaction, improving the signal to noise ratio.