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PacBio’s ability to produce extremely long reads makes it a useful complement to Illumina. Indeed, PacBio machines are better at sequencing “confusing” genomes, such as those with many copies of the same gene, long stretches of repetitive motifs, and “structural variations” like large insertions or deletions, which may not show up in short-read sequences. For instance, PacBio was used to sequence a very difficult bacterium called Clostridium autoethanogenum, which contains repeats, nine copies of a single gene, and insertions from bacterial virus genomes — basically the genomic equivalent of a Thomas Pynchon novel.
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Friedmann began appealing his conviction in 2023. Last summer, the appeal was denied. In December, the Tennessee Supreme Court declined to review the case. Friedmann is now in the Bledsoe County Correctional Complex, in Pikeville, where he is still writing. He has been contributing to the online magazine of the Prison Journalism Project, covering minor injustices, such as the comparatively high cost that his Muslim prisonmates have to pay for Qurans. He has not lost his sense of humor. Recently, he wrote an essay called “The Art of the Prison Fart.” In 2022, his play “Twenty Years in Solitary” earned him another first-place award from PEN America.